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Minicourse EDTH-SC13

Probing cerebral perfusion:
diffuse correlation spectroscopy and beyond

Stefan Carp

About this course

Changes in cerebral perfusion are hallmarks of both functional brain activation and pathological changes. Diffuse correlation spectroscopy (DCS) and related methods offer the possibility to directly monitor tissue perfusion non-invasively by quantifying the fluctuations of light diffusely reflected from tissue, fluctuations predominantly driven by the dynamic light scattering from moving red blood cells. We will present the theoretical basis of DCS and describe practical DCS system design and operation. We will also review advanced forms of DCS that offer substantial increases in measurement performance, enabling fast acquisition with high signal-to-noise, including long-wavelength operation, heterodyne approaches and simultaneous multi-speckle detection. We will also demonstrate data analysis, using both analytical and Monte-Carlo models.

Learning Outcomes

  • Become familiar with diffuse correlation spectroscopy (DCS) as a tool for measuring cerebral blood flow
  • Employ DCS technology and understand its principles of operation.

Course Plan

Level: Introductory

Pre-Requisites: None
Course Duration: 2 hours

Delivery Plan

60 min: presentation
10 min: break
30 min: data analysis demonstration
20 min: questions and answers

Why enrol on this course?

The goal of the course is to help participants become familiar with diffuse correlation spectroscopy as a tool for measuring cerebral blood flow. Cerebral blood flow (CBF) changes are the immediate result of neural activation and offer a potentially stronger and faster signal for functional imaging. CBF alterations also reflect brain pathological processes in a clinical setting.

After attending this course participants will be able to employ DCS technology and understand its principles of operation. We will describe the fundamental principles behind blood flow measurements using diffuse correlation spectroscopy, the key components of a DCS system, ways to increase measurement performance by modifying system design, methods of data analysis, including advanced multi-layer modeling, and limitations of current systems and areas of future technology development.